A disrupted FOXP3 transcriptional signature underpins systemic regulatory T cell insufficiency in early pregnancy failure

A disrupted FOXP3 transcriptional signature underpins systemic regulatory T cell insufficiency in early pregnancy failure

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Summary: Regulatory T (Treg) cell defects are implicated in disorders of embryo implantation and placental development, but the origins of Treg cell dysfunction are unknown.Here, we comprehensively analyzed the phenotypes and transcriptional profile of peripheral blood Treg cells in individuals with early pregnancy failure (EPF).Compared to fertile subjects, EPF subjects had 32% fewer total Treg cells and 54% samsung galaxy tab s7 fe 12.4 in. bundle, 64gb black fewer CD45RA+CCR7+ naive Treg cells among CD4+ T cells, an altered Treg cell phenotype with reduced transcription factor FOXP3 and suppressive marker CTLA4 expression, and lower Treg:Th1 and Treg:Th17 ratios.

RNA sequencing demonstrated an aberrant gene expression profile, with upregulation of pro-inflammatory genes arm tray for wheelchair including CSF2, IL4, IL17A, IL21, and IFNG in EPF Treg cells.In silico analysis revealed 25% of the Treg cell dysregulated genes are targets of FOXP3.We conclude that EPF is associated with systemic Treg cell defects arising due to disrupted FOXP3 transcriptional control and loss of lineage fidelity.